Golden Gate Assembly of CRISPR gRNA expression array for simultaneously targeting multiple genes.
Vad-Nielsen J, Lin L, Bolund L, Nielsen AL, Luo Y
Cell Mol Life Sci. 2016 May 13.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80784 | pMA-SpCas9-g1 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array |
| 80785 | pMA-SpCas9-g2 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array |
| 80786 | pMA-SpCas9-g3 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array |
| 80787 | pMA-SpCas9-g4 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array |
| 80788 | pMA-SpCas9-g5 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array |
| 80789 | pMA-SpCas9-g6 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array |
| 80790 | pMA-SpCas9-g7 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array |
| 80791 | pMA-SpCas9-g8 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array |
| 80792 | pMA-SpCas9-g9 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array |
| 80793 | pMA-SpCas9-g10 | BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array |
| 80794 | pMA-MsgRNA-EGFP | For insertion of gRNA array containing 11-30 gRNA modules |