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Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments.

Yoo TY, Choi JM, Conway W, Yu CH, Pappu RV, Needleman DJ
Elife. 2018 Jul 25;7. pii: 36392. doi: 10.7554/eLife.36392. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
80760pJAG98-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
80761pBABE-puro-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
80762pBABE-hygro-mTurquoise2-Nuf2Express Nuf2 N-terminally labeled with mTurquoise2
83285pTY07human CENP-A N-terminally labeled with LSSmOrange
83286pJAG98-mTurquoise2-FHA2-AURKB substrate-YPetuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

Antibodies from Article