TNF-insulin crosstalk at the transcription factor GATA6 is revealed by a model that links signaling and transcriptomic data tensors.
Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger DA, Janes KA
Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72607 | pBabe 3XFLAG-wtGATA6-3XAU1 puro | Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells |
| 72608 | pBabe 3XFLAG-S37A-GATA6-3XAU1 puro | For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation |
| 72609 | pBabe 3XFLAG-T34A-GATA6-3XAU1 puro | For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation |
| 72610 | pBabe 3XFLAG-S33A-GATA6-3XAU1 puro | For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation |
| 72611 | pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro | For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37 |
| 72612 | pEN_TT 3XFLAG-wtGATA6-3XAU1 | Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6 |
| 72613 | pEN_TT 3XFLAG-S37A-GATA6-3XAU1 | Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6 |
| 72615 | tet-pLKO shGATA6 puro | Expresses an inducible short hairpin targeting human GATA6 sequence |
| 72616 | pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant | Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6 |
| 72617 | pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant | Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6 |
| 72618 | pSLIK 3XFLAG-wtGATA6-3XAU1 neo | Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6 |
| 72922 | pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant | Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6 |
| 72924 | pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant | Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6 |