ALIX-CHMP4 interactions in the human ESCRT pathway.
McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 10.1073/pnas.0801567105. Epub 2008 May 29.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
80630 | GST-CHMP4A (pGEX2T(TEV)) | Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197 |
80632 | GST-CHMP4A (1-204) (pGEX2T(TEV)) | Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198 |
80633 | GST-CHMP4A (205-222) (pGEX2T(TEV)) | Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60 |
80634 | GST-CHMP4B (205-224) (pGEX2T(TEV)) | Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201 |
80635 | GST-CHMP4C (216-233) (pGEX2T(TEV)) | Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202 |
80637 | GST-CHMP4A (205-222) W220A (pGEX2T(TEV)) | Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203 |
80638 | GST-CHMP4A (205-222) L217A (pGEX2T(TEV)) | Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204 |
80639 | GST-CHMP4A (205-222) L214A (pGEX2T(TEV)) | Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205 |
80640 | GST-CHMP4A (205-222) E209A (pGEX2T(TEV)) | Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206 |
80641 | pet16b ALIX Bro1 (1-359) | Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428 |
80642 | pet151D-TOPO ALIX Bro1-V (1-702) | Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648 |