Controlling mRNA stability and translation with the CRISPR endoribonuclease Csy4.
Borchardt EK, Vandoros LA, Huang M, Lackey PE, Marzluff WF, Asokan A
RNA. 2015 Nov;21(11):1921-30. doi: 10.1261/rna.051227.115. Epub 2015 Sep 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80591 | TR-GFP | Expresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |
| 80592 | TR- ATG-HP-GFP | Expresses GFP with an Csy4 target hairpin inserted immediately following the start codon for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |
| 80594 | TR-5'UTR-HP-GFP | Expresses GFP from the CBA promoter. Includes a Csy4 target hairpin in the 5'UTR for Csy4-mediated regulation. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |
| 80601 | TR-Csy4 | Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |
| 80602 | TR-Csy4-H29A | Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |
| 80696 | TR-Csy4-R115A/R119A | Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV. |