Design and implementation of a biomolecular concentration tracker.
Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. Epub 2014 May 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80392 | pWW2149 | Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains. |
| 80393 | pVH028 | Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut) |
| 80394 | pVH001 | Contains aTc inducible Scaffold-mCherry-AAV and TetR genes |
| 80395 | pVH011 | Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry and TetR genes. Used for Western blots. |
| 80396 | pVH002 | pTet-Scaffold with no fluorescent protein. Used as a control for autofluorescence |
| 80397 | pVH003 | Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments |
| 80398 | pVH015 | Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots |
| 80399 | pVH009 | Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV. |
| 80400 | pVH016 | No anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG). |
| 80401 | pVH004 | Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence. |