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Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster.

Lin CC, Potter CJ
Genetics. 2016 Jun 22. pii: genetics.116.191783. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
80274pHACK-QF2QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs
80275pHACK-GAL4>QF2GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.
80276pBPGUw-HACK-QF2QF2 HACK backbone compatible with GMR attP integrated insertions.
80277pBPGUw-HACK-G4>QF2GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.
104873pHACK-GAL4>QF2(newHA1)HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Antibodies from Article