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delta-COP contains a helix C-terminal to its longin domain key to COPI dynamics and function.

Arakel EC, Richter KP, Clancy A, Schwappach B
Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6916-21. doi: 10.1073/pnas.1603544113. Epub 2016 Jun 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73863p415 CHI-1Chimaera 1 (Ret2-COPD)
73869p415 CHI-7Chimaera 7 (COPD-Ret2)
73871pRS303 Bt δCOPBos taurus Arcn1
73872p415 Ret2Ret2 (full length)
73873P415 Ret2LD2αRet2LD2α - His tagged (μHD deleted)
73874p415 δCLD2αδCLD2α - His tagged (μHD deleted)
73875P415 Ret2LDRet2LD - His tagged (2α helices+μHD deleted)
73876p415 δCLDδCLD - His tagged (2α helices+μHD deleted)
73877P415 Ret2LD+δC2α Swapped helices
73878P415 δCLD+Ret2 2αSwapped helices
73879p415 CHI-9Chimaera 9
73880p415 CHI-10Chimaera 10
73881p415 CHI-12Chimaera 12
73882p415 CHI-13Chimaera 13
73883p415 CHI-14Chimaera 14
73884p415 CHI-15Chimaera 15
73887p415 Ret2 I149AI149A mutation
73888p415 Ret2 I155AI155A mutation
73920pGEX6P1- Mst7-Erp1CTGST fusion- Erp1 cytoplasmic tail
73921pGEX6P1-GCN4cc-Erp1CTGST fusion- coiled coil- Erp1 cytoplasmic tail
73922pGEX6P1- Mst7-Erp2CTGST fusion- Erp2 cytoplasmic tail
73923pGEX6P1-GCN4cc-Erp2CTGST fusion- coiled coil- Erp2 cytoplasmic tail
75259p415 Ret2LD2a Q146FQ146F mutation
75260p415 Ret2LD2a N152TN152T mutation
75261p415 Ret2LD2a K153QK153Q mutation
75262p415 Ret2LD2a T158KT158K mutation

Antibodies from Article