A split horseradish peroxidase for the detection of intercellular protein-protein interactions and sensitive visualization of synapses.

Martell JD, Yamagata M, Deerinck TJ, Phan S, Kwa CG, Ellisman MH, Sanes JR, Ting AY
Nat Biotechnol. 2016 May 30. doi: 10.1038/nbt.3563. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
79914	pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1	piggyBac transposon vector, Zeocin-2A-sHRPbNLG1
79913	pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta	piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta
79912	pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1	piggyBac transposon vector, Cerulean-2A-sHRPbNLG1
79910	pXL-CAG-ICeuI-sHRPaNRX3beta	piggyBac transposon vector, sHRPaNRX3beta
73152	pCTCON2 Aga2P-HRP	Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.
73151	YIP sHRPa-Aga1P	Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
73150	pDisplay sHRPb-KDEL	Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
73149	pDisplay sHRPa-KDEL	Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.
73148	Synapsin sHRPb-NLG in FSW lentiviral vector	Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
73147	Synapsin sHRPa-NRX in FSW lentiviral vector	Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.