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Molecular recordings by directed CRISPR spacer acquisition.

Shipman SL, Nivala J, Macklis JD, Church GM
Science. 2016 Jun 9. pii: aaf1175. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
79879pWUKI 1+2Encodes Cas1+2 and a CRISPR array
80088pRSF-DUET Cas1 Cas2Encodes Cas1 and Cas2
80089pWURA Cas1Encodes Cas1
80090pWUR Cas2Encodes Cas2
80091pWUKI Cas1 Cas2 E9QEncodes Cas1 and Cas2 E9Q
80092pWUKI Cas1 V134L Cas2Encodes Cas1 V134L and Cas2
80093pWUKI Cas1 V33A Cas2Encodes Cas1 V33A and Cas2
80094pWUKI Cas1 V190I Cas2Encodes Cas1 V190I and Cas2
80095pWUKI Cas1 P279L Cas2Encodes Cas1 P279L and Cas2
80096pWUKI Cas1 P202Q Cas2Encodes Cas1 P202Q and Cas2
80097pWUKI Cas1 P212L Cas2Encodes Cas1 P212L and Cas2
80098pWUKI Cas1 P282L Cas2Encodes Cas1 P282L and Cas2
80099pWUKI Cas1 P294H Cas2Encodes Cas1 P294H and Cas2
80100pWUKI Cas1 Cas2 T72IEncodes Cas1 and Cas2 T72I
80101pWUKI Cas1 Cas2 G74AEncodes Cas1 and Cas2 G74A
80102pWUR 1+2 tetO mut89Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

Antibodies from Article