Molecular recordings by directed CRISPR spacer acquisition.
Shipman SL, Nivala J, Macklis JD, Church GM
Science. 2016 Jun 9. pii: aaf1175.
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PubMed
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
79879 | pWUKI 1+2 | Encodes Cas1+2 and a CRISPR array |
80088 | pRSF-DUET Cas1 Cas2 | Encodes Cas1 and Cas2 |
80089 | pWURA Cas1 | Encodes Cas1 |
80090 | pWUR Cas2 | Encodes Cas2 |
80091 | pWUKI Cas1 Cas2 E9Q | Encodes Cas1 and Cas2 E9Q |
80092 | pWUKI Cas1 V134L Cas2 | Encodes Cas1 V134L and Cas2 |
80093 | pWUKI Cas1 V33A Cas2 | Encodes Cas1 V33A and Cas2 |
80094 | pWUKI Cas1 V190I Cas2 | Encodes Cas1 V190I and Cas2 |
80095 | pWUKI Cas1 P279L Cas2 | Encodes Cas1 P279L and Cas2 |
80096 | pWUKI Cas1 P202Q Cas2 | Encodes Cas1 P202Q and Cas2 |
80097 | pWUKI Cas1 P212L Cas2 | Encodes Cas1 P212L and Cas2 |
80098 | pWUKI Cas1 P282L Cas2 | Encodes Cas1 P282L and Cas2 |
80099 | pWUKI Cas1 P294H Cas2 | Encodes Cas1 P294H and Cas2 |
80100 | pWUKI Cas1 Cas2 T72I | Encodes Cas1 and Cas2 T72I |
80101 | pWUKI Cas1 Cas2 G74A | Encodes Cas1 and Cas2 G74A |
80102 | pWUR 1+2 tetO mut89 | Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2 |