A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.

Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
79873	pJMP1	Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA
79874	pJMP2	Bacillus subtilis sgRNA expression vector; integrates into amyE
79875	pJMP3	Bacillus subtilis sgRNA expression vector; integrates into thrC