An E3-ligase-based method for ablating inhibitory synapses.
Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, Arnold DB
Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79869 | pCAG_EGFP-GFE3 | Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses. |
| 79870 | pCAG_EGFP-RandE3 | Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3. |
| 79871 | pAAV-Ef1a-DIO_EGFP-GFE3 | For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner. |
| 79872 | pAAV-Ef1a-DIO_EGFP-RandE3 | For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner. |