EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont PS
ACS Synth Biol. 2016 May 2.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72934 | pTU1 | Backbone vector with internal BsaI removed by site-directed mutagenesis |
| 72935 | pTU1-A-lacZ | Level 1 Destination vector - Position A |
| 72936 | pTU1-B-lacZ | Level 1 Destination vector - Position B |
| 72937 | pTU1-C-lacZ | Level 1 Destination vector - Position C |
| 72938 | pTU1-D-lacZ | Level 1 Destination vector - Position D |
| 72939 | pTU1-A-RFP | Level 1 Destination vector - Position A |
| 72940 | pTU1-B-RFP | Level 1 Destination vector - Position B |
| 72941 | pTU1-C-RFP | Level 1 Destination vector - Position C |
| 72942 | pTU1-D-RFP | Level 1 Destination vector - Position D |
| 72943 | pTU1-D1-RFP | Level 1 Destination vector - Position D1 |
| 72944 | pTU1-E-RFP | Level 1 Destination vector - Position E |
| 72945 | pTU3-A-RFP | Level 3 Destination vector - Assembly of 2 Level 2 modules - AB |
| 72946 | pTU3-B-RFP | Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD |
| 72947 | pBP | Backbone vector with internal BsmBI removed by site-directed mutagenesis |
| 72948 | pBP-lacZ | Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning |
| 72949 | pBP-ORF | Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site |
| 72950 | pTU2-A-lacZ | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72951 | pTU2-B-lacZ | Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72952 | pTU2-C-lacZ | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72953 | pTU2-D-lacZ | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72954 | pTU2-A-RFP | Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72955 | pTU2-B-RFP | Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72956 | pTU2-C-RFP | Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72957 | pTU2-D-RFP | Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE |
| 72958 | pTU2-a-RFP | Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB |
| 72959 | pTU2-b-RFP | Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC |
| 72960 | pBP-ORF-eGFP | Level 0 Entry vector" ORF - eGFP " |
| 72961 | pBP-ORF-eCFP | Level 0 Entry vector" ORF - eCFP" |
| 72962 | pBP-ORF-mCherry | Level 0 Entry vector" ORF - mCherry " |
| 72963 | pBP-J23100 | Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
| 72964 | pBP-J23108 | Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
| 72965 | pBP-J23114 | Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion) |
| 72966 | pBP-SJM901 | Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion) |
| 72967 | pBP-SJM902 | Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion) |
| 72968 | pBP-SJM903 | Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion) |
| 72969 | pBP-SJM905 | Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion) |
| 72970 | pBP-SJM906 | Level 0 - SJM906 promoter (5' CTAT/3' GTAC fusion) |
| 72971 | pBP-SJM908 | Level 0 - SJM908 promoter (5' CTAT/3' GTAC fusion) |
| 72972 | pBP-SJM910 | Level 0 - SJM910 promoter (5' CTAT/3' GTAC fusion) |
| 72973 | pBP-SJM911 | Level 0 - SJM911 promoter (5' CTAT/3' GTAC fusion) |
| 72974 | pBP-SJM912 | Level 0 - SJM912 promoter (5' CTAT/3' GTAC fusion) |
| 72975 | pBP-SJM914 | Level 0 - SJM914 promoter (5' CTAT/3' GTAC fusion) |
| 72976 | pBP-SJM915 | Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion) |
| 72977 | pBP-T7 | T7 promoter (5' CTAT/3' GTAC fusion) |
| 72978 | pBP-T7_RBS | T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion) |
| 72979 | pBP-T7_RBS-His6-Thrombin | T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion) |
| 72980 | pBP_BBa_B0034 | Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion) |
| 72981 | pBP-pET-RBS | PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion) |
| 72982 | pBP-Tag_linker | N-terminal linker with pET-RBS (5' GTAC/3' TAAA fusion) |
| 72983 | pBP-HexHis | Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion) |
| 72984 | pBP-Strep(II) | Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion) |
| 72985 | pBP-TL1 | RBS_H1 |
| 72986 | pBP-TL2 | RBS_H2 |
| 72987 | pBP-TL3 | RBS_H3 |
| 72988 | pBP-TL4 | RBS_H5 |
| 72989 | pBP-TL5 | RBS_M2 |
| 72990 | pBP-TL6 | RBS_M3 |
| 72991 | pBP-TL7 | RBS_M5 |
| 72992 | pBP-TL8 | RBS_L1 |
| 72993 | pBP-TL9 | RBS_L2 |
| 72994 | pBP-TL10 | RBS_L3 |
| 72995 | pBP-TL11 | RBS_L4 |
| 72996 | pBP-TL12 | RBS_L5 |
| 72997 | pBP-BBa_B0012 | BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion) |
| 72998 | pBP-BBa_B0015 | BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion) |
| 72999 | pBP-L3S2P21 | L3S2P21 terminator |
| 73000 | pBP-L3S1P51 | L3S1P51 terminator |
| 73001 | pBP-L3S1P32 | L3S1P32 terminator |
| 73002 | pBP-L3S1P11 | L3S1P11 terminator |
| 73003 | pBP-L2U8H11 | L2U8H11 terminator |
| 73004 | pBP-L2U5H11 | L2U5H11 terminator |
| 73005 | pBP-L2U5H08 | L2U5H08 terminator |
| 73006 | pBP-L2U3H03 | L2U3H03 terminator |
| 73007 | pBP-L2U2H09 | L2U2H09 terminator |
| 73008 | pBP-L1U1H09 | L1U1H09 terminator |
| 73009 | pBP-L1U1H08 | L1U1H08 terminator |
| 73010 | pTU2_2a | Secondary module (BpiI) site in pTU2a backbone |
| 73011 | pTU2_2b | Secondary module (BpiI) site in pTU2b backbone |
| 74088 | pTU2S-a (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication |
| 74089 | pTU2S-b (p15A origin) | Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication |
| 74090 | pTU2-A-RFP (p15A origin) | pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication |
| 74091 | pTU2S-a (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication |
| 74092 | pTU2S-b (colE1 origin) | Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication |
| 74093 | pTU2-A-RFP (colE1 origin) | pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication |
| 74094 | pBP-Bba_K822000 | L-Lactate inducible promoter from E. coli MG1655 |
| 74095 | pBP-lldR | L-lactate repressor (LldR) from E. coli MG1655 |
| 74096 | pBP-T7RNAP | T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA |
| 74097 | pBP-BBa_J23100 | BBa_J23100 promoter (iGEM collection) |
| 74098 | pBP-TL9 | TL9 RBS (AAAAAG) |