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CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii.

Li Q, Chen J, Minton NP, Zhang Y, Wen Z, Liu J, Yang H, Zeng Z, Ren X, Yang J, Gu Y, Jiang W, Jiang Y, Yang S
Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73228pNICKclos2.0Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
73639pNICKclos1.0Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
73640pdCASclosTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

Antibodies from Article