Long-range transcriptional interference in E. coli used to construct a dual positive selection system for genetic switches.
Hoffmann SA, Kruse SM, Arndt KM
Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. Epub 2016 Feb 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79824 | pSAH0016 | Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol |
| 79826 | pBAD_Kan-LacI | Arabinose induced expression of Lac repressor |
| 79828 | SAH0141-0.0-conP01 | Transcriptional interference assessment; strong interfering promoter, landing site for spacers |
| 79830 | SAH0098-scrPX | Transcriptional interference assessment; no interfering promoter (scrambled) |
| 79831 | SAH0146-0.5-conP01 | Transcriptional interference assessment; strong interfering promoter, 500 bp spacer |