An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing.
Gao X, Chen J, Dai X, Zhang D, Zhao Y
Plant Physiol. 2016 May 15. pii: pp.00663.2016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78931 | pHDE-35S-Cas9-mCherry | For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants |
| 78932 | pHDE-35S-Cas9-mCherry-UBQ | For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs |