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Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells.

Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P, Johnson JD
Mol Metab. 2016 Feb 10;5(5):366-78. doi: 10.1016/j.molmet.2016.01.009. eCollection 2016 May. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
79791InsRA-TagBFPInsulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
79795InsRA-eGFPInsulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
79797InsRA-EBFP2Insulin receptor isoform A with a inter-domain EBFP2 tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
79799pTag-BFP-C-h-Rab4a-c-MycExpress TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).
79800pTag-RFP-C-h-Rab4a-c-MycExpress TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).
79801pTag-BFP-C-h-Rab5a-c-MycExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).
79802pTag-RFP-C-h-Rab5a-c-MycExpress TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).
79803pTag-BFP-C-h-Rab7L1-c-MycExpress TagBFP labeled human Rab7L1.
79804pTag-RFP-C-h-Rab7L1-c-MycExpress TagRFP labeled human Rab7L1.
79805pTag-BFP-C-h-Rab11aExpress TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).
79806pTag-RFP-C-h-Rab11aExpress TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Antibodies from Article