Mextli proteins use both canonical bipartite and novel tripartite binding modes to form eIF4E complexes that display differential sensitivity to 4E-BP regulation.
Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, Weichenrieder O, Igreja C, Izaurralde E
Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. Epub 2015 Aug 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79262 | pAc5.1B-EGFP-DmMextli | Expresses EGFP-tagged DmMextli in S2 cells |
| 148337 | pAc5.1B-EGFP-DmMextli_577-620_AB | Insect Expression of DmMextli_577-520 |
| 148338 | pAc5.1B-EGFP-DmMextli_577-640_AB | Insect Expression of DmMextli_577-640 |
| 148339 | pAc5.1B-lambdaN-HA-DmeIF4E-N110EH111E-siRNAres_AB | Insect Expression of DmeIF4E-N110EH111E-siRNAres |
| 148340 | pAc5.1B-lambdaN-HA-DmMextli-F625E_AB | Insect Expression of DmMextli-F625E |
| 148341 | pAc5.1B-lambdaN-HA-DmMextli-F625EY630E_AB | Insect Expression of DmMextli-F625EY630E |
| 148342 | pAc5.1B-lambdaN-HA-DmMextli-I612AL613A_AB | Insect Expression of DmMextli-I612AL613A |
| 148343 | pAc5.1B-lambdaN-HA-DmMextli-L598D_AB | Insect Expression of DmMextli-L598D |
| 148344 | pAc5.1B-lambdaN-HA-DmMextli-M605D_AB | Insect Expression of DmMextli-M605D |
| 148345 | pAc5.1B-lambdaN-HA-DmMextli-W596D_AB | Insect Expression of DmMextli-W596D |
| 148346 | pAc5.1B-lambdaN-HA-DmMextli-W602D_AB | Insect Expression of DmMextli-W602D |
| 148347 | pAc5.1B-lambdaN-HA-DmMextli-Y581AL587AL596A_AB | Insect Expression of DmMextli-Y581AL587AL596A |
| 148348 | pAc5.1B-lambdaN-HA-DmMextli-YLLILtoAAAAA_AB | Insect Expression of DmMextli-YLLILtoAAAAA |
| 148349 | pnEA-NpM-CeeIF4G_315-491opt_AB | Bacterial Expression of CeeIF4G_315-491opt |
| 148350 | pnEA-NpM-CeMextli_471-507opt_AB | Bacterial Expression of CeMextli_471-507opt |
| 148351 | pnEA-NpM-CeMextli_471-507opt-GB1_AB | Bacterial Expression of CeMextli_471-507opt |
| 148352 | pnEA-NpM-CeMextli_471-507opt-I497DI504D-GB1_AB | Bacterial Expression of CeMextli_471-507opt-I497DI504D |
| 148353 | pnEA-NpM-CeMextli_471-507opt-M493D-GB1_AB | Bacterial Expression of CeMextli_471-507opt-M493D |
| 148354 | pnEA-NpM-CeMextli_471-507opt-Y479AL484AM485A-GB1_AB | Bacterial Expression of CeMextli_471-507opt-Y479AL484AM485A |
| 148355 | pnEA-NpM-DmMextli_577-620_AB | Bacterial Expression of DmMextli_577-620 |
| 148356 | pnEA-NpM-DmMextli_577-620-GB1_AB | Bacterial Expression of DmMextli_577-620 |
| 148357 | pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB | Bacterial Expression of DmMextli_577-620-I583RS590R |
| 148358 | pnEA-NpM-DmMextli_577-620-I612AL613A-GB1_AB | Bacterial Expression of DmMextli_577-620-I612AL613A |
| 148359 | pnEA-NpM-DmMextli_577-620-Y581AL586AL587A-GB1_AB | Bacterial Expression of DmMextli_577-620-Y581AL586AL587A |
| 148360 | pnEA-NpM-DmMextli_577-640_AB | Bacterial Expression of DmMextli_577-640 |
| 148361 | pnEA-NpM-DmMextli_577-640-F625EL631E-GB1_AB | Bacterial Expression of DmMextli_577-640-F625EL631E |
| 148362 | pnEA-NpM-DmMextli_577-640-GB1_AB | Bacterial Expression of DmMextli_577-640 |
| 148363 | pnEA-NpM-DmMextli_577-640-I612AL613A-GB1_AB | Bacterial Expression of DmMextli_577-640-I612AL613A |
| 148364 | pnEA-NpM-DmMextli_577-640-M605D-GB1_AB | Bacterial Expression of DmMextli_577-640-M605D |
| 148365 | pnEA-NpM-DmMextli_577-640-Y581AL586AL587A-GB1_AB | Bacterial Expression of DmMextli_577-640-Y581AL586AL587A |
| 148366 | pnEA-NpM-DmMextli_577-640-YLLILtoAAAAA-GB1_AB | Bacterial Expression of DmMextli_577-640-YLLILtoAAAAA |
| 148367 | pnYC-NpH-CeeIF4E3_1-215opt_AB | Bacterial Expression of CeeIF4E3_1-215 |
| 148368 | pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB | Bacterial Expression of CeeIF4E3_1-215-V58AI74A |
| 148369 | pnYC-NpH-CeeIF4E3_30-215opt_AB | Bacterial Expression of CeeIF4E3_30-215opt |