GW182 proteins cause PABP dissociation from silenced miRNA targets in the absence of deadenylation.
Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 2013 Mar 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79258 | pAc5.1B-EGFP-DmeIF4E | Expresses EGFP-tagged DmeIF4E in S2 cells |
| 79259 | pAc5.1B-lambdaN-HA-DmeIF4G-V5His6 | Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells |
| 146188 | pAc5.1B-lambdaN-HA-DmeIF4E_E | Insect Expression of DmeIF4E |
| 147097 | pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_O | Insect Expression of DmPABPC1-siRNAres |
| 147333 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1CIM2mut_Q | Insect Expression of HsTNRC6C_1207-1690-CIM1CIM2mut |
| 147334 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1CIM2mut-F1389A_Q | Insect Expression of HsTNRC6C_1207-1690-CIM1CIM2mut-F1389A |
| 147335 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM1mut_Q | Insect Expression of HsTNRC6C_1207-1690-CIM1mut |
| 147336 | pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM2mut_Q | Insect Expression of HsTNRC6C_1207-1690-CIM2mut |
| 147337 | pAc5.1B-lambdaN-HA-HsTNRC6C-F1389A_Q | Insect Expression of HsTNRC6C-F1389A |
| 147550 | pAc5.1B-EGFP-HsTNRC6C_1207-1609-M2WA_T | Insect Expression of HsTNRC6C_1207-1609-M2WA |
| 147551 | pAc5.1B-EGFP-HsTNRC6C_1207-1609-M2WA-CIM1-2mut_T | Insect Expression of HsTNRC6C_1207-1609-M2WA-CIM1-2mut |
| 147756 | pAc5.1B-MS2-DmPABPC1-V5His6_V | Insect Expression of DmPABPC1 |
| 147757 | pAc5.1B-MS2-GST-V5His6_V | Insect Expression vector |
| 147758 | pAc5.1C-Fluc-5BoxB-6xbsMS2-HhR_V | Insect Expression of 5BoxB-6xbsMS2-HhR |
| 147759 | pAc5.1C-Fluc-5BoxB-PolyA-HhR_V | Insect Expression of 5BoxB-PolyA-HhR |
| 148385 | pAc5.1C-Fluc-A93-5BoxB-HhR_AC | Insect Expression of A93-5BoxB-HhR |