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Usefulness of a Darwinian system in a biotechnological application: evolution of optical window fluorescent protein variants under selective pressure.

Schoetz U, Deliolanis NC, Ng D, Pauli J, Resch-Genger U, Kuhn E, Heuer S, Beisker W, Koster RW, Zitzelsberger H, Caldwell RB
PLoS One. 2014 Sep 5;9(9):e107069. doi: 10.1371/journal.pone.0107069. eCollection 2014. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
79537pRSETa-Amrose v1pRSETa (Invitrogen) plasmid expressing Amrose variant 1
79538pRSETa-Amrose v2pRSETa (Invitrogen) plasmid expressing Amrose variant 2
79539pRSETa-Amrose v3pRSETa (Invitrogen) plasmid expressing Amrose variant 3

Antibodies from Article