Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors.
Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA
Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 79119 | pHR_5x Gal4 UAS | Base vector for cloning Gal4DBDVP64 inducible genes |
| 79120 | pHR_PGK | Lentiviral vector for constitutive expression of genes (PGK promoter) |
| 79121 | pHR_SFFV | Lentiviral vector for constitutive expression of genes (SFFV promoter) |
| 79123 | pHR_Gal4UAS_IRES_mC_pGK_tBFP | Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors |
| 79124 | pHR_Gal4UAS_pGK_mCherry | Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES) |
| 79125 | pHR_PGK_antiCD19_synNotch_Gal4VP64 | Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter) |
| 79126 | pHR_PGK_antiCD19_synNotch_TetRVP64 | Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter) |
| 79127 | pHR_pGK_LaG17_synNotch_Gal4VP64 | Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter) |
| 79128 | pHR_SFFV_LaG17_synNotch_TetRVP64 | Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter) |
| 79129 | pHR_EGFPligand | Lentiviral vector for surface expressed EGFP (SFFV promoter) |
| 79130 | pHR_Gal4UAS_tBFP_PGK_mCherry | Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry |