A simple DNA handle attachment method for single molecule mechanical manipulation experiments.
Min D, Arbing MA, Jefferson RE, Bowie JU
Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 2016 Jun 6.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
79053 | pMBP-SpyCatcher | Expresses maltose binding protein (MBP) SpyCatcher fusion |
79054 | pSpyTag-GlpG | Expresses SpyTagged GlpG protein |