Programmable RNA-binding protein composed of repeats of a single modular unit.

Adamala KP, Martin-Alarcon DA, Boyden ES
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2579-88. doi: 10.1073/pnas.1519368113. Epub 2016 Apr 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
87319	PumA	PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
87320	PumG	PumHD w/each unit mutated to bind G (as in Fig. 1C; unit 6 is the Pumby for G)
87321	PumC	PumHD w/each unit mutated to bind C (as in Fig. 1C; unit 6 is the Pumby for C)
87322	PumU	PumHD w/each unit mutated to bind U (as in Fig. 1C; unit 6 is the Pumby for U)
97189	[UBC][EIF4e][GG cloning]	EIF4e plasmid with Pum cloning site
97190	[UBC][firefly luciferase][target cloning][renilla luciferase]	Reporter for translation activation via Pum.
97191	[GG][Pin]	Pin nuclease domain with Pum cloning site
97192	[UBC][NRE Pum][eGFP]	The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.
97193	[Bla][IRES][GFP]	Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
97194	[GFP][IRES][Bla]	Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.