Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation.
McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA
Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78254 | TetO-dCas9-D3A | Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter |
| 78255 | TetO-dCas9-mD3A | Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter |
| 78256 | pCMV-dCas9-D3A | Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter |
| 78257 | pCMV-dCas9-mD3A | Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter |