Phase separation of signaling molecules promotes T cell receptor signal transduction.
Su X, Ditlev JA, Hui E, Xing W, Banjade S, Okrut J, King DS, Taunton J, Rosen MK, Vale RD
Science. 2016 Apr 29;352(6285):595-9. doi: 10.1126/science.aad9964. Epub 2016 Apr 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78505 | GST-PS-Grb2 (XSB363) | Construct for expressing Grb2 in E. Coli |
| 78506 | GST-PS-Sos1 (PRM) - Cys (XSB364) | Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling |
| 78507 | GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365) | Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling |
| 78508 | His10-CD3z-SNAPf-ybbR (XSB507) | Construct for expressing CD3z-SNAPf-ybbR in E. Coli |
| 78509 | CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316) | Construct for expressing CD45R0 (extracellular domain) His10 in Sf9 |
| 78510 | GST-PS-Grb2 1-154 (XSB382) | Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli |
| 78512 | His10-PS-SNAPf (XSB522) | Construct for expressing His10 - SNAPf in E. Coli |
| 78513 | His10-PS-SNAPf-9R (XSB525) | Construct for expressing His10 - SNAPf -9R in E. Coli |
| 78514 | His10-PS-SNAPf-9E (XSB526) | Construct for expressing His10 - SNAPf -9E in E. Coli |
| 78515 | pHR-LAT-Y6F YYYY-mCitrine (XSB586) | Lentiviral vector for expressing LAT-Y6F YYYY-mCitrine (3 Grb2 binding sites) |
| 78516 | pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588) | Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites) |
| 78517 | PHR-LAT Y6F YFFF-mCitrine (XSB589) | Lentiviral vector for expressing LAT-Y6F YFFF-mCitrine (0 Grb2 binding sites) |