Consequences of Cas9 cleavage in the chromosome of Escherichia coli.

Cui L, Bikard D
Nucleic Acids Res. 2016 Apr 8. pii: gkw223. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
78492	pCRRNAcos	Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.
78493	pZA31-sulA-GFP	Expression of GFP under the sulA promoter.
78494	pLC13	Expression of Mu-gam under the pBAD promoter
78551	LC-E02	E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.
78552	LC-E03	E. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.