Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells.
Sladitschek HL, Neveu PA
PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78175 | MXS_BidirectionalCAG | Bidirectional CAG promoter (compatible with the MXS Chaining Kit) |
| 82477 | miReporter-PGK | microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette. |
| 82478 | miReporter-CAG | microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette. |