Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein.

Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, Lambowitz AM, Fire AZ
Science. 2016 Feb 26;351(6276):aad4234. doi: 10.1126/science.aad4234. (Link opens in a new window) PubMed (Link opens in a new window) Article

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ID	Plasmid	Purpose
78409	pV92.2	pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct