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Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters.

Aymoz D, Wosika V, Durandau E, Pelet S
Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
74247pDA183pSTL1-dPSTRr(2,1)
74248pDA200pGPD1-dPSTRy(4,3)
74249pDA125Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).
74250pDA186Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).
74251pDA171Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).
74275pDA199pSTL1-dPSTRy(4,3)

Antibodies from Article