Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9.
Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78621 | pX330_HR_Prnp_3 | pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence |
| 78622 | pX335_HR_Prnp_3 | pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence |
| 78623 | pX459_HR_Prnp_3 | pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence |
| 78624 | sgRNA(MS2)_Prnp_SAM1 | Encodes sgRNA2.0 targeting 158nt upstream of TSS |
| 78625 | sgRNA(MS2)_Prnp_SAM2 | Encodes sgRNA2.0 targeting 138nt upstream of TSS |