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Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.

Magaraci MS, Bermudez JG, Yogish D, Pak DH, Mollov V, Tycko J, Issadore D, Mannickarottu SG, Chow BY
ACS Synth Biol. 2016 Apr 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
78688BC-A1-001A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
78689BC-A1-002A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
78690BC-A1-003A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
78691BC-A1-004A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.
78692BC-A1-005A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
78693BC-A1-006A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
78694BC-A1-007A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
78695BC-A1-008A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.
78696BC-A1-009A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
78697BC-A1-010A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.
78699BC-A1-011A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
78701BC-A1-013A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
78702BC-A1-014A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.
78703BC-A1-015A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
78704BC-A1-016A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

Antibodies from Article