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Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.

Vancraenenbroeck R, Kunzelmann S, Webb MR
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
78206pTEV5_ RpMatB_2Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
78207pTEV5_ RpMatB_3Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding
78208pTEV5_ RpMatB_4Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

Antibodies from Article