Development of a range of fluorescent reagentless biosensors for ATP, based on malonyl-coenzyme A synthetase.
Vancraenenbroeck R, Kunzelmann S, Webb MR
PLoS One. 2017 Jun 21;12(6):e0179547. doi: 10.1371/journal.pone.0179547. eCollection 2017.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
78206 | pTEV5_ RpMatB_2 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding |
78207 | pTEV5_ RpMatB_3 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding |
78208 | pTEV5_ RpMatB_4 | Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding |