Dynamics of epigenetic regulation at the single-cell level.
Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, Elowitz MB
Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78099 | PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins | Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B). |
| 78101 | pEx1-pEF-H2B-mCherry-T2A-rTetR-EED | Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells. |
| 78348 | pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB | Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells. |
| 78349 | pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4 | Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells. |
| 78352 | pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo | Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells. |
| 78353 | pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo | Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells. |