Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase.
Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75366 | CIB81-pmGFP | Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end |
| 75367 | CIB1-CreC(N1) | Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase |
| 75368 | CRY2(L348F)-CreN | Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase |
| 75369 | Gal4BD-CRY2(535) | Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535) |
| 75370 | CRY2PHR(W349R)-mCherry | Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry |
| 75372 | CRY2(535, L348F)-mCherry | Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry |