Structural basis for histone H2B deubiquitination by the SAGA DUB module.
Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C
Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75299 | His6TEV-UbG76C | Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease |
| 75332 | H2BK120C | Bacteria expression of H2BK120C |
| 118217 | pTXB1-xlH2B(1-114)_K114A | Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa. |
| 118252 | pTXB1-H2B(1-114)_wt | Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa |