CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing.

Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP
Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
74119	AIO-GFP	All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
74120	AIO-mCherry	All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
74630	AIO-Puro	All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing