NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.
Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, Ulevitch RJ, Beutler B
Nat Immunol. 2016 Mar;17(3):250-8. doi: 10.1038/ni.3333. Epub 2015 Dec 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75127 | pcDNA3-N-Flag-NLRP3 | Expresses N-terminal Flag-tagged NLRP3 in mammalian cells |
| 75128 | pcDNA3-N-Flag-Caspase-1 | Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells |
| 75131 | pCMV-pro-Il1b-C-Flag | Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells |
| 75134 | pcDNA3-N-Flag-ASC1 | Expresses N-terminal Flag-tagged ASC1 in mammalian cells |
| 75137 | pcDNA3-N-Flag-NLRP3 1-90 | Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells |
| 75140 | pcDNA3-N-Flag-NLRP3 91-710 | Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells |
| 75141 | pcDNA3-N-Flag-NLRP3 711-1034 | Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells |
| 75142 | pcDNA3-N-HA-NEK7 | Expresses N-terminal HA-tagged NEK7 in mammalian cells |
| 75143 | pcDNA3-N-HA-NEK7 K64M | Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells |