A general strategy for the evolution of bond-forming enzymes using yeast display.
Chen I, Dorr BM, Liu DR
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 10.1073/pnas.1101046108. Epub 2011 Jun 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 75144 | sortase A pentamutant (eSrtA) in pET29 | evolved sortase A (eSrtA) pentamutant with improved kinetics and activity |