Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR-Cas9 genome-editing efficiency.
Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 2017 Nov 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74939 | pcDNA3-Flag::UbvG08 I44A, deltaGG | Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1 |
| 74940 | pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG | Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant) |
| 75030 | pMX-HA-UbvG08-IRES-GFP | Retroviral vector with ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53) |
| 75031 | pMX-HA-UbvG08_MUT-IRES-GFP | Retroviral vector with ubiquitin variant with reverted mutations L69P and V70L (i53-DM mutant) |
| 92170 | pAAV-i53 | recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1 |
| 92171 | pAAV-i53-DM | recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM) |