LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate.
Belluzzi E, Gonnelli A, Cirnaru MD, Marte A, Plotegher N, Russo I, Civiero L, Cogo S, Carrion MP, Franchin C, Arrigoni G, Beltramini M, Bubacco L, Onofri F, Piccoli G, Greggio E
Mol Neurodegener. 2016 Jan 13;11(1):1. doi: 10.1186/s13024-015-0066-z.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74924 | pcDNA3-Flag-NSF | Mammalian expression of human NSF full-length (744 aa) |
| 74936 | pcDNA3-Flag-NSF/T645A | Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein) |
| 74937 | pcDNA3-Flag-NSF/T646A | Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein) |
| 74938 | pcDNA3-Flag-NSF/S647A | Mammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein) |