In vivo gene editing in dystrophic mouse muscle and muscle stem cells.
Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY, Xiao R, Ran FA, Cong L, Zhang F, Vandenberghe LH, Church GM, Wagers AJ
Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177. Epub 2015 Dec 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78601 | pZac2.1 SV40-CMV-SaCas9-3xNLS | AAV vector containing SaCas9 |
| 78603 | pZac2.1 U6-SaDMDR7-U6-SaDMDL2 | AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23 |
| 78604 | pZac2.1 U6-SaAi9L-U6-SaAi9R | AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette |
| 78606 | pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2 | pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2 |
| 78607 | pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2 | A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23 |
| 78608 | pCRII-Topo U6-SpAi9L-U6SpDMDL | Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22 |
| 78609 | pCRII-Topo U6-SpAi9R-U6-SpDMDR | Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23 |