A Ras-like domain in the light intermediate chain bridges the dynein motor to a cargo-binding region.
Schroeder CM, Ostrem JM, Hertz NT, Vale RD
Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74597 | pGEX6P1-human full-length LIC1 | used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag |
| 74598 | pGEX6P1-human LIC1 G domain | used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389 |
| 74599 | pGEX6P1-human LIC1 C-terminal half | used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523 |
| 74600 | pET28a-RILP | used for expression in E. coli and purification |
| 74601 | pHR-mCherry-p2A-full length LIC1 | expresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherry |
| 74602 | pHR-mCherry-p2A-LIC1 G domain | expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry |
| 74603 | pHR-mCherry-p2A-LIC1 C-terminal half | expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry |
| 74605 | pHR-mCherry-p2A-LIC1 Y101A, Y103A | expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein |
| 74606 | pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A | expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein |
| 74753 | pET28a-FIP3 | Expressed sfGFP-tagged FIP3 in E. coli for protein production |
| 74780 | pGEX6P1-C. thermophilum LIC | encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum |
| 74782 | pET21a-Chaetomium thermophilum LIC | encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag |