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Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids.

Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance DM, Hahn J, Koffas MA
Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73392pETM6-At4CL-PhCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73393pETM6-At4CL-PhCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73394pETM6-At4CL-CmCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73395pETM6-At4CL-CmCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73396pETM6-Pc4CL-PhCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73397pETM6-Pc4CL-PhCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73398pETM6-Pc4CL-CmCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73399pETM6-Pc4CL-CmCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73400pETM6-Vv4CL-PhCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73401pETM6-Vv4CL-PhCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73402pETM6-Vv4CL-CmCHS-MsCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73403pETM6-Vv4CL-CmCHS-CmCHIExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivo
73404pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Antibodies from Article