Versatile protein tagging in cells with split fluorescent protein.
Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70217 | pEGFP-GFP11-Clathrin light chain | Expresses GFP11-clathrin light chain in mammalian cells |
| 70218 | pACUH-GFP11x7-mCherry-α-tubulin | Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths. |
| 70219 | pcDNA3.1-GFP(1-10) | Expresses GFP(1-10) in mammalian cells |
| 70220 | pEGFP-sfCherry11x4-ß-actin | Expresses sfCherry11x4-ß-actin in mammalian cells |
| 70221 | pEGFP-sfCherry11-ß-actin | Expresses sfCherry11-ß-actin in mammalian cells |
| 70222 | pcDNA3.1-sfCherry(1-10) | Expresses sfCherry(1-10) in mammalian cells |
| 70224 | pHRm-NLS-dCas9-GFP11x7-NLS | Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells |
| 70228 | pHRm-GFP(1-10)-VP64-NLS | Expresses GFP(1-10)-VP64-NLS in mammalian cells |
| 80409 | pHR-SFFV-GFP1-10 | Expresses GFP1-10 in mammalian cells |