Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, Zoldos V
Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71666 | pdCas9-DNMT3A-EGFP | Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA |
| 71667 | pdCas9-DNMT3A-PuroR | Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA |
| 71684 | pdCas9-DNMT3A-PuroR (ANV) | Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA |
| 71685 | pdCas9-DNMT3A-EGFP (ANV) | Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA |
| 71828 | pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 | Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control |
| 71829 | pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV) | Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control |
| 71830 | pdCas9-DNMT3A-PuroR_hNT | Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells |
| 74407 | pdCas9-DNMT3A-PuroR_v2 | Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA |
| 74408 | pdCas9-DNMT3A-PuroR (ANV)_v2 | Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA |