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Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates

Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
bioRxiv 041871 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
74368pMOD6-pGRR-r11-r10-yeGFPpGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.
74369pMOD8A-iRGR-r11This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
74370pMOD8A-RGR-r10This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

Antibodies from Article