Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates
Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
bioRxiv 041871
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
74368 | pMOD6-pGRR-r11-r10-yeGFP | pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function. |
74369 | pMOD8A-iRGR-r11 | This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function. |
74370 | pMOD8A-RGR-r10 | This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function. |