Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.
Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, Steinmetz LM, Parts L, St Onge RP
Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73796 | pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA | pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA |