Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex.
Balasubramani A, Larjo A, Bassein JA, Chang X, Hastie RB, Togher SM, Lahdesmaki H, Rao A
Nat Commun. 2015 Jun 22;6:7307. doi: 10.1038/ncomms8307.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74244 | pCMV6-XL4 ASXL1 (1-1304) | Cancer-associated ASXL1 truncation mutant in mammalian expression vector |
| 74245 | pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG | Cancer-associated ASXL1 truncation mutant in mammalian expression vector |
| 74261 | pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG | Cancer-associated ASXL1 truncation mutant in mammalian expression vector |
| 74262 | pCMV6-XL4 ASXL1 (1-479) 3x FLAG | Cancer-associated ASXL1 truncation mutant in mammalian expression vector |
| 74263 | pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG | Cancer-associated ASXL1 truncation mutant in mammalian expression vector |
| 81021 | MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG | Cancer-associated ASXL1 truncation mutant in retroviral vector |
| 81022 | MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG | Cancer-associated ASXL1 truncation mutant in retroviral vector |
| 81023 | MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG | Cancer-associated ASXL1 truncation mutant in retroviral vector |
| 81024 | BAP1 | Retroviral vector containing human BAP1 and Thy1.1 selection marker |
| 81025 | BAP1 C91A | Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker |