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Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting.

Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J
Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
54538dClover2-N1Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
54577dClover2-C1Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
74234pNCS-mRuby3Constitutive expression of mRuby3 in bacteria
74236pNCS-mClover3Constitutive expression of mClover3 in bacteria
74252pKanCMV-mClover3-mRuby3Mammalian expression of tandem fusion of mClover3-mRuby3
74253pKanCMV-mClover3-18aa-TubulinMammalian expression of N-terminal fusion of mClover3 to Tubulin
74254pKanCMV-CX4.3-7aa-mRuby3Mammalian expression of C-terminal fusion of mRuby3 to CX43
74255pKanCMV-mRuby3-18aa-actinMammalian expression of N-terminal fusion of mRuby3 to actin
74256pKanCMV-mRuby3-18aa-TubulinMammalian expression of N-terminal fusion of mRuby3 to Tubulin
74257pKanCMV-mClover3-10aa-H2BMammalian expression of N-terminal fusion of mClover3 to H2B
74258pKanCMV-mRuby3-10aa-H2BMammalian expression of N-terminal fusion of mRuby3 to H2B.
74259pKanCMV-mClover3-18aa-actinMammalian expression of N-terminal fusion of mClover3 to actin
74260pKanCMV-CX4.3-7aa-mClover3Mammalian expression of C-terminal fusion of mClover3 to CX43

Antibodies from Article