An improved smaller biotin ligase for BioID proximity labeling.
Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ
Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74223 | myc-BioID2-MCS | To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag |
| 74224 | MCS-BioID2-HA | To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag |
| 80899 | MCS-13X Linker-BioID2-HA | To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags. |
| 80900 | mycBioID2-pBABE-puro | To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tag |
| 92308 | mycBioID2-13X Linker-MCS | To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags. |